Transcriptomic analysis of chondroprogenitors in response to flow fluid shear stress / 实用口腔医学杂志
Journal of Practical Stomatology
; (6): 36-42, 2024.
Article
в Zh
| WPRIM
| ID: wpr-1020534
Ответственная библиотека:
WPRO
ABSTRACT
Objective:To investigate the response of mandibular condylar chondroprogenitors to flow fluid shear stress(FFSS).Methods:Chondroprogenitors were in vitro cultured and stimulated with FFSS that can cause cell degeneration,and treated with sec-ond-generation high-throughput RNA sequencing.Differential gene expression was screened using DESeq2 software for gene ontology(GO)functional enrichment analysis,kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis and protein-protein interaction(PPI)network analysis.qRT-PCR was performed to validate the core genes screened by PPI.Results:A total of 1996 differentially expressed genes were obtained,mainly including inflammatory response and cell cycle related molecules.Among them,Actal,Atf3,Ccl2,116,Nfkbia,Ret and Vcaml were identified as the core genes.Conclusion:FFSS stimulation affects chondroprogenitor function by acting on inflammatory responses and cell cycle-related signaling pathways in chondroprogenitors.
Полный текст:
1
База данных:
WPRIM
Язык:
Zh
Журнал:
Journal of Practical Stomatology
Год:
2024
Тип:
Article