Lesion striatal extracts from 6-hydroxydopamine-induced PD rats induce the differentiation of BMSCs / 中华神经医学杂志

ABSTRACT

Objective To explore the possibility of BMSCs differentiating into neurons under thc condition of in vitro culture by striatal extracts (SEs) from 6-hydroxydopamine (6-OHDA)-induced PD rats. Methods PD rat models were established by stereotaxic injection of 6-OHDA into the right medial forebrain bundle. Bilateral striatum was dissected; lesion striatal extracts (L-SEs) and intact striatal extracts (I-SEs) were prepared. The in vitro isolated and expanded third-passage BMSCs were induced by the 2 striatal extracts, respectively. Cells were observed under phase contrast microscope. The differentiated cells were detected and measured by immunocytochemical stain with nestin, neuron special enolase (NSE), glial fibrillary acidic protein (GFAP) and tyroxine hydroxylase (TH). Besides that, some other BMSCs were cultured in the serumal culture solution (Ser-C) and free serum culture solution (F-SerC) as controls. Results Morphological changes of BMSCs after treatment could be observed under contrast phase microscope. Some cells even had enations and some cells in the L-SEs and I-SEs inducement groups were out of shape. Cells in the Ser-C treatment group grew well, while cells in F-SerC treatment group could not grow well. Immunohistochemical staining indicated that GFAP positive cells expressed in the Ser-C treatment group; the GFAP and nestin positive cells in the L-SEs inducement group were increased and the GFAP and NSE positive cells in the L-SEs inducement group were increased as compared with those in the Ser-C treatment group; furthermore, the percentages of GFAP positive cells in the L-SEs and I-SEs inducement groups (53.09±32.27%, 54.60±33.14%) were significantly different with those in the Ser-C treatment group (15.05±3.92%, P＜0.05) and those were not obviously different between the L-SEs and I-SEs inducement groups (P＞0.05). No TH positive cells existed in all the groups. Conclusion SEs from 6-OHDA-induced PD rats can lead to morphological changes of BMSCs, and some cells have enations. A certain number of cells express neural special proteins after inducemet, including GFAP positive cells and some nestin/NSE positive cells.