Study on antidepressant mechanism of helicid based on network pharmacology / 安徽医科大学学报
Acta Universitatis Medicinalis Anhui
; (6): 1896-1901, 2022.
Article
в Zh
| WPRIM
| ID: wpr-1038689
Ответственная библиотека:
WPRO
ABSTRACT
Objective @#To screen the target of helicid in the intervention of depression based on network pharmacol- ogy and molecular docking,and to study the effect of helicid on the expression level of related targets in hippocam- pus,prefrontal cortex,striatum and habenular nucleus of chronic unpredictable mild stress ( CUMS) rats.@*Methods @#The target of helicid was predicted by SwissTargetPrediction database ,and the depression related targets were screened by GeneCards、DisGeNet、TTD and DRUGBANK databases ; the metascape platform was used for gene en- richment analysis ,and the " helicid-depression-pathway " network was constructed ; Autodock Vina was used for molecular docking research ; qRT-PCR was used to detect the effect of helicid on the mRNA expression of HTR1A, ADORA1 and ADORA2A in rat tissues. @*Results @#The 81 helicid targets and 1 640 depression targets were ob- tained,including 40 intersecting targets ; the key targets were mainly enriched in cAMP signal pathway,PI3K-Akt signal pathway,MAPK signal pathway and so on ; the results of molecular docking showed that the binding activity of helicid with most targets was good ; helicid up-regulated the expression levels of HTR1A ,ADORA1 and ADORA2A mRNA in hippocampus,prefrontal cortex ,striatum and habenular nucleus of CUMS rats.@*Conclusion@#Helicid may act on cAMP,PI3K-Akt,MAPK and other signal pathways to intervene depression through HTR1A, ADORA1 and ADORA2A.
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База данных:
WPRIM
Язык:
Zh
Журнал:
Acta Universitatis Medicinalis Anhui
Год:
2022
Тип:
Article