The Mycobacterium avium subsp. paratuberculosis fibronectin attachment protein, a toll-like receptor 4 agonist, enhances dendritic cell-based cancer vaccine potency
Experimental & Molecular Medicine
; : 340-349, 2012.
Article
в En
| WPRIM
| ID: wpr-153073
Ответственная библиотека:
WPRO
ABSTRACT
In this study, we showed the direct interaction between Mycobacterium avium subsp. paratuberculosis fibronectin attachment protein (FAP) and toll-like receptor4 (TLR4) via co-localization and binding by using confocal microscopy and co-immunoprecipitation assays. FAP triggered the expression of pro- and anti-inflammatory cytokines in a TLR4-dependent manner. In addition, FAP-induced cytokine expression in bone marrow-derived dendritic cells (BMDCs) was modulated in part by glycogen synthase kinase-3 (GSK-3). FAP-induced expression of CD80, CD86, major histocompatibility complex (MHC) class I, and MHC class II in TLR4+/+ BMDCs was not observed in TLR4-/- BMDCs. Furthermore, FAP induced DC-mediated CD8+ T cell proliferation and cytotoxic T lymphocyte (CTL) activity, and suppressed tumor growth with DC-based tumor vaccination in EG7 thymoma murine model. Taken together, these results indicate that the TLR4 agonist, FAP, a potential immunoadjuvant for DC-based cancer vaccination, improves the DC-based immune response via the TLR4 signaling pathway.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Paratuberculosis
/
Protein Binding
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Thymoma
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Dendritic Cells
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T-Lymphocytes, Cytotoxic
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Signal Transduction
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Gene Expression Regulation
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Cytokines
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CD8-Positive T-Lymphocytes
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Adhesins, Bacterial
Пределы темы:
Animals
/
Humans
Язык:
En
Журнал:
Experimental & Molecular Medicine
Год:
2012
Тип:
Article