Effect of CD14, Toll-like receptors, cytoskeletal inhibitors and NF-kappaB inhibitor on MMP-8 release from human neutrophils induced by E. coli lipopolysaccharides / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
; : 427-436, 2005.
Article
в Ko
| WPRIM
| ID: wpr-204289
Ответственная библиотека:
WPRO
ABSTRACT
OBJECTIVE: MMP-8 is a neutrophil enzyme and its level increases in some inflammatory diseases, including periodontal disease. We knew that the lipopolysaccharide of E.coli(E-LPS) induced MMP-8 release from human neutrophils. E-LPS is known to induce the production and release of inflammatory cytokines through CD14, Toll-like receptor(TLR). In the present study, we investigated whether MMP-8 release by E-LPS is induced via CD14-TLR pathway and the cellular mechanism of MMP-8 release in human neutrophils. MATERIAL AND METHODS: Human neutrophils were isolated from the peripheral blood of healthy donors and pre-incubated in medium containing antibodies against CD14, anti-TLR2 and anti-TLR4 or several inhibitors of microtubules and microfilaments and then incubated with E-LPS. The cells were treated TPCK and E-LPS simultaneously. The MMP-8amount in the culture medium was determined using ELISA. RESULTS: E-LPS increased MMP-8release from neutrophils and its induction was inhibited by anti- CD14 and anti-TLR4 but not by anti-TLR2 antibodies. The inhibitors of micro- tubule and microfilament polymerization significantly decreased E-LPS-induced MMP- 8release. TPCK inhibited E-LPS-induced MMP-8 release. CONCLUSION: These results suggest that MMP-8 release is induced by E-LPS via the CD14-TLR4 signal pathway in human neutrophils and may be depedent on microtubule and microfilament systems and NF-kappaB pathway.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Periodontal Diseases
/
Polymers
/
Tissue Donors
/
Tosylphenylalanyl Chloromethyl Ketone
/
Actin Cytoskeleton
/
Enzyme-Linked Immunosorbent Assay
/
Signal Transduction
/
Lipopolysaccharides
/
Cytokines
/
NF-kappa B
Пределы темы:
Humans
Язык:
Ko
Журнал:
The Journal of the Korean Academy of Periodontology
Год:
2005
Тип:
Article