Identification of Mycobacteria Species by HPLC and Species Distribution during Five Years at Ulsan University Hospital / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
; : 24-33, 2008.
Article
в Ko
| WPRIM
| ID: wpr-219032
Ответственная библиотека:
WPRO
ABSTRACT
BACKGROUND: Infections caused by mycobacteria have been significantly increasing. Due to the difficulty of making a decision about the pathogenicity of mycobacteria, species-level identification is very important for patients' diagnosis and treatment. The purpose of this study was to identify mycobacteria species using a high performance liquid chromatography (HPLC) method and to provide an initial database for the distribution of mycobacteria in Korea. METHODS: Acid fast bacteria isolated from 3,107 clinical specimens were identified by mycolic acid analysis using HPLC. The HPLC patterns were compared with those of standard mycobacteria species. RESULTS: The HPLC patterns were divided into single, double, and triple cluster groups, each group comprising 9, 20, and 4 species, respectively. Mycobacteria and non-tuberculous mycobacteria (NTM) were identifies by HPLC at the rates of 99.5% and 95.6%, respectively. NTM was isolated in 12.4% of the mycobacteria positive specimens. This study also found that there were 20 different NTM species with the distribution of each species ranging from 0.3% to 15.9% of the total NTM. While the rate of NTM has been increasing in Korea, M. avium-intracellulare, M. fortuitum, and M. chelonae are relatively decreasing, and M. kansasii and M. gordonae are relatively increasing. CONCLUSIONS: HPLC method was highly discriminative for the identification of NTM in clinical specimens.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Chromatography, High Pressure Liquid
/
Bacterial Typing Techniques
/
Hospitals, University
/
Korea
/
Nontuberculous Mycobacteria
/
Mycobacterium Infections, Nontuberculous
/
Mycolic Acids
Тип исследования:
Prognostic_studies
Пределы темы:
Humans
Страна как тема:
Asia
Язык:
Ko
Журнал:
The Korean Journal of Laboratory Medicine
Год:
2008
Тип:
Article