Inhibition of telomerase with hTERT antisense enhances TNF-alpha-induced apoptosis in prostate cancer cells PC3 / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the inhibiting effect of telomerase with hTERT antisense on TNF-alpha-induced apoptosis in prostate cancer cells PC3.</p><p><b>METHODS</b>Antisense phosphorothioate oligodeoxynucleotide (AS PS-ODN) was synthesized and purified. Telomerase activity was measured by telomeric repeat amplification protocol (TRAP) and telomerase PCR-ELISA Kit, cell viability was determined by MTT assay, and cell apoptosis was observed by morphological method and determined by flow cytometry.</p><p><b>RESULTS</b>AS PS-ODN could significantly inhibit the telomerase activity and increase the susceptibility of TNF-alpha-induced apoptosis of PC3 cells.</p><p><b>CONCLUSION</b>Inhibition of telomerase with hTERT antisense can enhance TNF-alpha-induced apoptosis in prostate cancer cells.</p>