ITS sequence of 9 Bupleurum species and its application in identification of Chaihu (Radix Buplurei) / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>The study the patterns of rDNA ITS sequence variation of 9 medicinal species of genus Bupleurum in China to find the DNA molecular markers for identification of these crude drugs.</p><p><b>METHOD</b>The ITS regions of these species were amplified by PCR and sequenced, and their sequences were analyzed by DNAssist Version 2.2.</p><p><b>RESULTS</b>Homologous alignment indicated that the consanguinity of the ITS sequences between genus Bupleurum and outgroups was lower than 75%, and that within genus Bupleurum was higher than 88%. For the same species, the consanguinity was higher than 99%.</p><p><b>CONCLUSION</b>The ITS sequences may serve as reliable molecular markers for identification of Radix Bupleuri.</p>