M gene analysis of canine coronavirus strains detected in Korea
Journal of Veterinary Science
; : 495-502, 2014.
Article
в En
| WPRIM
| ID: wpr-24551
Ответственная библиотека:
WPRO
ABSTRACT
The purpose of this study was to investigate the genetic features of canine coronavirus (CCV) strains detected in Korea. M gene sequences obtained for isolates from 22 dogs with enteritis over a 5-year period were evaluated. Sequence comparison revealed that the 22 Korean CCV strains had an 87.2 to 100% nucleotide homology. Comparing to the typical reference CCV strains (type II), the nucleotide sequence of Korean strains had homology ranged from 86.3% to 98.3% (89.1% to 99.2% for the amino acid sequence) and 87.7% to 97.8% (92.4% to 100% for the amino acid sequence) when compared to FCoV-like CCV strains (type I). Three amino acid variations in the M gene were characteristic for the Korean CCV strains. Phylogenetic analysis demonstrated that the 22 Korean CCV strains belonged to four typical CCV clusters (i.e., a unique Korean CCV cluster, a type II and transmissible gastroenteritis virus cluster, an intermediate cluster between type I and II, and a type I cluster). This study was the first to identify genetic differences of the M gene from Korean CCV strains and provided a platform for molecular identification of different Korean CCV strains.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Phylogeny
/
Molecular Sequence Data
/
Viral Matrix Proteins
/
Polymerase Chain Reaction
/
Amino Acid Sequence
/
Coronavirus, Canine
/
Coronavirus Infections
/
Reverse Transcriptase Polymerase Chain Reaction
/
Dog Diseases
/
Republic of Korea
Тип исследования:
Prognostic_studies
Пределы темы:
Animals
Страна как тема:
Asia
Язык:
En
Журнал:
Journal of Veterinary Science
Год:
2014
Тип:
Article