The significance of TGF-beta expression in scar in adenosine receptor A(2A) knockout mice / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To discuss the mechanism of scar hypertrophy in adenosine receptor A(2A) (A(2A) R) knockout mice.</p><p><b>METHODS</b>Animal models of hypertrophic scar were established in 12 A(2A) R knockout mice and 12 wild-type mice as control. The thickness and the size of transverse section of the hypertrophic scar were observed by H-E staining. The hydroxyproline (HYP) in the scar was measured colorimetrically. The TGF-beta expression was tested by Western blotting method.</p><p><b>RESULTS</b>The hypertrophic scar in wild-type mice was more severe than that in knockout mice. Compared with self-control, the increase of the thickness and the size of transverse section of hypertrophic scar was markedly higher in wild-type group than in the knockout group (P < 0.01). There was significant difference in HYP content between the two groups (P < 0.01). Compared with self-control, the increase of TGF-beta expression in wild-type group was much more than that in knockout group (P < 0.01).</p><p><b>CONCLUSIONS</b>The TGF-beta expression decreases in the A(2A) R knockout mice. The scar hypertrophy is also much less in the A(2A) R knockout mice.</p>