Study on the acute toxicity experiment of mice and anti-tumor function in vitro of the qinglongyi / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 887-890, 2004.
Article
в Zh
| WPRIM
| ID: wpr-272775
Ответственная библиотека:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the anti-tumor effect and acute toxicity in vitro of the separation compositions from qinglongyi.</p><p><b>METHOD</b>The conventional acute toxicity experiments of mice, the MTT [3-(4,5-dimethylthiazol-2 yl)-2,5-diphenylterazolium bromide, MTT] and the SRB (sulforhodamin B) were used to make preliminary selection to qinglongyi and its separation compositions.</p><p><b>RESULT</b>The half-deadly dose (LD50, the half-lethiferous dose) of the chloroform separation composition in qinglongyi was 575.38 mg x kg(-1) (i.g.), and of the acetic ether separation compositions in qinglongyi was 1303.59 mg x kg(-1) (i.g.). From the other parts of separation compositions in qinglongyi, the LD50 were more than 5 g x kg(-1). When acetic ether separation composition of qinglongyi was at 100 microg x mL(-1) the growth inhibitory rate (GIR) was < 50% to the leukaemia cell HL-60 of human. When chloroform separation composition and the acetic ether separation compositions of qinglongyi was at 100 mg x mL(-1), GIR was 52% to the leukaemia cell HL-60, the gastric carcinoma cell BGC-823 and the cervical carcinoma cell Hela of human.</p><p><b>CONCLUSION</b>The separation composition of the chloroform and the acetic ether from qinglongyi have obvious, anti-tumor effect.</p>
Полный текст:
1
База данных:
WPRIM
Основная тема:
Pathology
/
Pharmacology
/
Plants, Medicinal
/
Stomach Neoplasms
/
Behavior, Animal
/
Drugs, Chinese Herbal
/
HeLa Cells
/
Chemistry
/
HL-60 Cells
/
Inhibitory Concentration 50
Пределы темы:
Animals
/
Female
/
Humans
/
Male
Язык:
Zh
Журнал:
China Journal of Chinese Materia Medica
Год:
2004
Тип:
Article