Determination of glycosides in traditional Chinese medicine liu-wei di-huang by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a RP-HPLC method for determination of glycosides in Traditional Chinese Medicine Liu-wei Di-huang.</p><p><b>METHOD</b>The samples were analyzed on an ODS column at 30 degrees C, with mobile phase of methanol/water (3367) at flow rate 1.0 mL.min and detection at wavelength of 236 nm.</p><p><b>RESULT</b>Three major components reached base-line separation and were identified to be mononiside, loganin, paeoniflorin. Respectively for the three components, linear correlations were found between peak areas and concentrations in the ranges of 7.4-60, 7.7-62 mg.L-1 and 8.5-68 mg.L-1, and the recoveries were 98.8%, 98.3%, 99.6%.</p><p><b>CONCLUSION</b>The established method is proved to be suitable for simultaneous quantification of three major glycosidic components in Liuwei Dihuang decoction and can be used for evaluation of the quality of Liuwei Dihuang preparations.</p>