Study on chromatographic fingerprint of extractive from Tripterygium wilfordii with supercritical carbon dioxide / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 706-709, 2007.
Article
в Zh
| WPRIM
| ID: wpr-283401
Ответственная библиотека:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish the chromatographic fingerprint of supercritical carbon dioxide extract of Tripterygium wilfordii.</p><p><b>METHOD</b>HPLC method was applied for quality assessment of T. Wilfordii, HPLC analysis was performed on Kromasil C18 (4. 6 mm x 250 mm, 5 microm) with the mixture of acetonitrile-1% per thousand H3PO4, as mobile phase in gradient mode. The samples were detected at UV of 267 nm with column temperature of 35 degrees C, analytic time was 80 min; Flow-rate was 1.0 mL x min(-1). The chromatographic fingerprint of ten batches of samples was determined, for establishing the chromatographic fingerprint of T. Wilfordii.</p><p><b>RESULT</b>Indicating 27 peaks in common, identified 21 peaks with chemical reference and HPLC-MS, and the HPLC fingerprint was established.</p><p><b>CONCLUSION</b>The method is steady and accurate with a good repeatability and can be used as a quality control method for T. Wilfordii.</p>
Полный текст:
1
База данных:
WPRIM
Основная тема:
Plants, Medicinal
/
Carbon Dioxide
/
Plant Extracts
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Plant Roots
/
Spectrometry, Mass, Electrospray Ionization
/
Tripterygium
/
Chromatography, Supercritical Fluid
Тип исследования:
Prognostic_studies
Язык:
Zh
Журнал:
China Journal of Chinese Materia Medica
Год:
2007
Тип:
Article