Effect of two Chinese medicinal compounds, blood-activating and water-draining medicine, on tumor necrosis factor α and nuclear factor κ B expressions in rats with intracerebral hemorrhage / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 857-864, 2014.
Article
в En
| WPRIM
| ID: wpr-289686
Ответственная библиотека:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of blood-activating Chinese medicinal compounds and water-draining Chinese medicinal compounds on tumor necrosis factor alpha (TNF-α) and nuclear factor kappaB (NF-κ B) expressions in rats with intracerebral hemorrhage (ICH) at the acute stage, and to monitor their therapeutic effect and mechanism of action on inflammation and cerebral edema.</p><p><b>METHODS</b>A rat model of cerebral hemorrhage was achieved by injecting autologous arterial blood into the caudate nucleus. A total of 168 rats were randomly divided into 4 groups: blood-activating medicine group (n=42), water-draining medicine group (n=42), sham operated group (n=42), and the model group (n=42). A series of brain samples were obtained at days 1, 3 and 5 after ICH from rats in all groups. Protein expression levels of TNF-α and NF-κ B were measured by immunohistochemical staining and gene expression levels of TNF-α and NF-κ B were measured by real-time fluorescent PCR.</p><p><b>RESULTS</b>Compared to the sham operated group, protein expression levels of TNF-α and NF-κ B in the model group significantly increased (P<0.01). Protein and gene expressions of TNF-α from the blood-activating medicine group and water-draining medicine group significantly decreased when compared to those in the model group P<0.05). Meanwhile, compared to the model group, the expression of NF-κ B in the blood-activating medicine group significantly decreased (P<0.05), while expression of NF-κ B in the water-draining medicine group did not differ (P>0.05).</p><p><b>CONCLUSIONS</b>Blood-activating Chinese medicinal compounds and water-draining Chinese medicinal compounds can alleviate inflammation of peripheral tissue and cerebral edema. However, the blood-activating Chinese medicinal compounds were more effective than the water-draining Chinese medicinal compounds. The possible effective mechanism may be by means of inhibiting the activation of NF-κ B so as to suppress the transcription of target genes including gene expression of TNF-α.</p>
Полный текст:
1
База данных:
WPRIM
Основная тема:
Blood
/
Body Water
/
Base Sequence
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NF-kappa B
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Tumor Necrosis Factor-alpha
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Rats, Sprague-Dawley
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DNA Primers
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Intracranial Hemorrhages
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Real-Time Polymerase Chain Reaction
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Medicine, Chinese Traditional
Тип исследования:
Diagnostic_studies
/
Prognostic_studies
Пределы темы:
Animals
Язык:
En
Журнал:
Chinese journal of integrative medicine
Год:
2014
Тип:
Article