Effect of imatinib at different concentrations on rat C6 glioma cell apoptosis and cell cycle / 南方医科大学学报
Journal of Southern Medical University
; (12): 1089-1091, 2010.
Article
в Zh
| WPRIM
| ID: wpr-289985
Ответственная библиотека:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of imatinib on rat C6 glioma cell apoptosis and cell cycle.</p><p><b>METHODS</b>MTT assay was used to determine the OD value of C6 glioma cells following treatment with imatinib at different concentrations (0.156, 10 and 15 micromo/L) for 24, 48 and 72 h. The cell apoptosis was assayed by Hochest/PI staining and the cell cycle changes were analyzed by flow cytometry.</p><p><b>RESULTS</b>Imatinib treatment resulted in increased number of apoptotic cells in a time- and dose-dependent manner. A 72-h treatment of the cells with imatinib at 10 and 15 micromo/L caused increased cell percentage in G(0)/G(1) phase to (68.53-/+0.83)% and (70.41-/+0.62)%, (P<0.01), decreased the percentage of G(2) phase cells to (14.48-/+0.12)% and (13.84-/+2.86)% (P<0.01), and decreased the percentage of S phase cells to (16.98-/+0.72)% and (15.78-/+2.28)%, respectively (P<0.01).</p><p><b>CONCLUSION</b>Imatinib can induce apoptosis and affect the distribution of the cell cycle of C6 cells in vitro.</p>
Полный текст:
1
База данных:
WPRIM
Основная тема:
Pathology
/
Pharmacology
/
Piperazines
/
Pyrimidines
/
Benzamides
/
Cell Cycle
/
Apoptosis
/
Cell Line, Tumor
/
Dose-Response Relationship, Drug
/
Imatinib Mesylate
Пределы темы:
Animals
Язык:
Zh
Журнал:
Journal of Southern Medical University
Год:
2010
Тип:
Article