Clinical study of human cytomegalovirus infection in colony forming unit-megakaryocyte in patients with idiopathic thrombocytopenic purpura / 中华儿科杂志
Chinese Journal of Pediatrics
; (12): 346-349, 2006.
Article
в Zh
| WPRIM
| ID: wpr-309203
Ответственная библиотека:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Idiopathic thrombocytopenic purpura (ITP) is a hemorrhagic disease in children with blood platelets redundant destruction caused by chaotic immunological mechanism. However, some patients with ITP with negative platelet-associated antibody and ineffective adrenal cortical hormone therapy probably have special pathogenesis. It is indicated that the human cytomegalovirus (HCMV) can incubate in haemopoietic stem cell/ancestral cell to inhibit its generation and differentiation. Therefore, the study was designed to investigate HCMV-late mRNA expression in megakaryoblast for the purpose of examining the pathogenesis of ITP and to examine the effectiveness of ganciclovir on ITP.</p><p><b>METHODS</b>Colony forming unit-megakaryocyte (CFU-MK) of 46 ITP patients with HCMV infection were incubated. Reverse transcription-polymerase chain reaction (RT-PCR) was subsequently used for HCMV-late mRNA detection. Ganciclovir therapy was given to both positive group and negative group for comparison of therapeutic effectiveness.</p><p><b>RESULTS</b>Nineteen out of 46 CFU-MK culture cell specimens with positive HCMV-DNA by PCR or positive CMV-IgM by enzyme linked immunosorbent assay (ELISA) from serum of peripheral blood showed positive for HCMV-late mRNA. While, the remaining 27 were negative. Sixteen positive responders to ganciclovir therapy were observed amongst those with positive HCMV-DNA. Whereas, only 4 positive responders to ganciclovir therapy were noticed amongst those with negative HCMV-DNA. The curative effectiveness in positive group was significantly higher than that in negative group (P < 0.01).</p><p><b>CONCLUSION</b>HCMV can directly infect CFU-MK, which might be one of the mechanisms responsible for ITP. Ganciclovir is an effective therapy resulting in an increase in thrombocyte in ITP patients whose HCMV-late mRNA was positive in their CFU-MK.</p>
Полный текст:
1
База данных:
WPRIM
Основная тема:
Antiviral Agents
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Virulence
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Blood Platelets
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Immunoglobulin M
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RNA, Messenger
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RNA, Viral
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Enzyme-Linked Immunosorbent Assay
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Ganciclovir
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Cells, Cultured
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Purpura, Thrombocytopenic, Idiopathic
Пределы темы:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
Язык:
Zh
Журнал:
Chinese Journal of Pediatrics
Год:
2006
Тип:
Article