Diagnostic value of SYT-SSX fusion gene detection by fluorescence in-situ hybridization for synovial sarcoma / 中华病理学杂志
Chinese Journal of Pathology
; (12): 660-664, 2008.
Article
в Zh
| WPRIM
| ID: wpr-315118
Ответственная библиотека:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method of SYT-SSX fusion gene detection by FISH and to explore its diagnostic value for synovial sarcoma.</p><p><b>METHODS</b>The presence of SYT-SSX fusion gene was determined by FISH using a tissue microarray containing 62 known synovial sarcomas, 60 non-synovial sarcomas and 133 equivocal synovial sarcomas. FISH results were compared with those of RT-PCR published previously.</p><p><b>RESULTS</b>Overall, 96.9% (247/255) of the cases were successfully analyzed by FISH. The sensitivity of FISH for known synovial sarcomas was 96.7% (58/60), and the specificity for the non-synovial sarcoma was 100% (59/59). Moreover, SYT-SSX gene fusion was detected in 78.1% (100/128) of the equivocal synovial sarcomas. The concordance rate between FISH and RT-PCR was 83.6% (102/122) in those equivocal synovial sarcomas, and overall 79.7% (106/133) of these cases were confirmed as synovial sarcomas either by RT-PCR or by FISH.</p><p><b>CONCLUSIONS</b>The sensitivity and specificity of FISH detection of SYT-SSX fusion gene are high. FISH and RT-PCR are complementary to each other in the confirmation of synovial sarcomas, particularly those questionable cases.</p>
Полный текст:
1
База данных:
WPRIM
Основная тема:
Soft Tissue Neoplasms
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Biomarkers, Tumor
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Oncogene Proteins, Fusion
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In Situ Hybridization, Fluorescence
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Sarcoma, Synovial
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Reverse Transcriptase Polymerase Chain Reaction
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Diagnosis
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Pathology, Molecular
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Genetics
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Methods
Тип исследования:
Diagnostic_studies
Пределы темы:
Humans
Язык:
Zh
Журнал:
Chinese Journal of Pathology
Год:
2008
Тип:
Article