Drug resistance and activity changes of thioredoxin reductase in pancreatic cancer cells strain SW1990 induced by gemcitabine / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 606-610, 2005.
Article
в Zh
| WPRIM
| ID: wpr-318854
Ответственная библиотека:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish gemcitabine-resistant pancreatic cancer cell strain and study the role of thioredoxin reductase (TrxR) in drug-resistant process.</p><p><b>METHODS</b>Gemcitabine-resistant pancreatic cancer cell strain SW1990/GZ was induced by increasing drug dosage intermittently, then the changes of its biological features and the activity of TrxR were examined.</p><p><b>RESULTS</b>Stable drug-resistant SW1990/GZ cell strain was established by culturing with gemcitabine for 9 months. The morphology and growth characteristics of the cell strain changed remarkably. The cells shrunk and became rounder; its endoplasm expanded; granular substances increased; and the doubling-time was prolonged. Resistance of the cell line to gemcitabine, fluorouracil, adriamycin, and mitomycin significantly increased. The TrxR activity of the drug-resistant cells was increased markedly.</p><p><b>CONCLUSION</b>SW1990/GZ has certain multidrug resistance to some chemotherapy drugs, and TrxR plays a role in the drug-resistant process.</p>
Полный текст:
1
База данных:
WPRIM
Основная тема:
Pancreatic Neoplasms
/
Pathology
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Pharmacology
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Thioredoxin-Disulfide Reductase
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Drug Resistance, Neoplasm
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Cell Line, Tumor
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Deoxycytidine
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Metabolism
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Antimetabolites, Antineoplastic
Пределы темы:
Humans
Язык:
Zh
Журнал:
Acta Academiae Medicinae Sinicae
Год:
2005
Тип:
Article