Effect of the C-terminal truncated human apoptosis-inducing factor δ1-480 on biological behaviors of MCF-7 cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1285-1288, 2013.
Article
в Zh
| WPRIM
| ID: wpr-319428
Ответственная библиотека:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the expression of the C-terminal truncated human apoptosis-inducing factor (AIF) and its biological effect on MCF-7 cells.</p><p><b>METHODS</b>PcDNA3.0-FDT-AIFδ1-480 was transfected into human breast carcinoma MCF-7 cells with lipofectamine. The expression of the truncated AIF gene was detected by Western blotting, and its effects on the biological behaviors of MCF-7 cells and on the expression of cytochrome c (cytC) were evaluated using flow cytometry, MTT assay, colony-forming assay, and mitochondrial membrane potential measurement.</p><p><b>RESULTS</b>PcDNA3.0-FDT-AIFδ1-480 enhanced AIF expression in MCF-7 cells, obviously inhibited the cell proliferation, and significantly reduced the mitochondrial membrane potentials (P<0.05). Transfection of the cells with PcDNA3.0-FDT-AIFδ1-480 promoted the expression of cytC and resulted in significantly increased apoptosis of MCF-7 cells (P<0.05).</p><p><b>CONCLUSION</b>The expression of C-terminal truncated human AIF gene can induce apoptosis of human MCF-7 cells by promoting cytC release from mitochondria.</p>
Полный текст:
1
База данных:
WPRIM
Основная тема:
Pathology
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Breast Neoplasms
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Apoptosis
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Cytochromes c
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Cell Proliferation
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Apoptosis Inducing Factor
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Membrane Potential, Mitochondrial
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MCF-7 Cells
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Genetics
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Metabolism
Пределы темы:
Female
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Humans
Язык:
Zh
Журнал:
Journal of Southern Medical University
Год:
2013
Тип:
Article