Effect of epidural block combined with general anesthesia on c-fos and HSP70 expression in the myocardium of spontaneous hypertensive rats / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the efficacy of epidural block combined with general anesthesia on stress reaction in spontaneous hypertensive rats (SHR).</p><p><b>METHODS</b>Eighteen SHR and 18 SD rats (above 24 weeks) were randomly divided into 6 groups, namely epidural block combined with general anesthesia group (A1,A2), general anesthesia group (B1,B2) and control group (D1,D2). General anesthesia was performed with celiac injection of droperidol, fentanyl and diazepam. Tracheal intubation and ventilation were performed after tracheotomy, and epidural block was conducted by incision. The rats in groups A1, A2, B1, B2 underwent splenectomy. All the rats were sacrificed 24 h after the surgery and two myocardium specimens were collected for detecting c-fos and HSP70 expression using RT-PCR.</p><p><b>RESULTS</b>c-fos mRNA expression was significantly lower but HSP70 mRNA expression significantly higher in group A1 than in group B1.</p><p><b>CONCLUSION</b>Compared with general anesthesia, general anesthesia combined with epidural block can reduce the stress responses and protect cardiac myocytes by decreasing c-fos expression and increasing HSP70 expression in SHR.</p>