Value of detecting p16 gene methylation in the diagnosis of malignant pleural effusion / 南方医科大学学报
Journal of Southern Medical University
; (12): 2148-2150, 2010.
Article
в Zh
| WPRIM
| ID: wpr-330758
Ответственная библиотека:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate aberrant methylation in the promoter of p16 gene in the sediment cells of pleural effusion and evaluate its clinical significance in the differentiating benign and malignant pleural effusion.</p><p><b>METHODS</b>Using methylation-specific PCR (MSP), aberrant promoter methylation of p16 gene was detected in the sedimental cells of pleural effusion samples from 66 patients with pleural effusion.</p><p><b>RESULTS</b>Of the 66 patients with pleural effusion, 36 had a definite diagnosis of malignant pleural effusion, and the rest were confirmed to have benign pleural effusion. The positivity rate of p16 gene promoter methylation was 69.4% (25/36) in malignant pleural effusion and 13.3% (4/30) in benign pleural effusion specimens, showing a significant difference between them (χ² = 20.915, P < 0.01). The diagnostic sensitivity, specificity and accuracy of aberrant promoter methylation of p16 gene in the 36 malignant cases were 69.4%, 86.7% and 77.3%, respectively. The positive expression of p16 gene promoter methylation in malignant pleural effusion was not correlated to the histological type or the pathological grade of the tumor (P > 0.05).</p><p><b>CONCLUSION</b>Detection of aberrant methylation in p16 gene promoter in the sediment cells of pleural effusion specimens by MSP method allows differentiation between benign and malignant pleural effusion.</p>
Полный текст:
1
База данных:
WPRIM
Основная тема:
Molecular Sequence Data
/
Base Sequence
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Pleural Effusion, Malignant
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Sensitivity and Specificity
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Promoter Regions, Genetic
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DNA Methylation
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Genes, p16
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Diagnosis
/
Genetics
Тип исследования:
Diagnostic_studies
Пределы темы:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
Язык:
Zh
Журнал:
Journal of Southern Medical University
Год:
2010
Тип:
Article