Clinical application of a method for evaluating von Willebrand factor cleaving protease activity / 南方医科大学学报
Journal of Southern Medical University
; (12): 1176-1179, 2007.
Article
в Zh
| WPRIM
| ID: wpr-337302
Ответственная библиотека:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for evaluating the activity of von Willebrand factor cleaving protease (vWF-cp) and evaluate its clinical application.</p><p><b>METHODS</b>Purified von Willebrand factor polymer was isolated by gel filtration from human fresh-frozen plasma as the enzyme substrate. SDS-PAGE, Western blotting, and luminographic detection were used to evaluate vWF-cp activity of 60 healthy adults, 28 patients with cerebral infarction (CI) and 7 with thrombotic thrombocytopenic purpura (TTP).</p><p><b>RESULTS</b>In the subjects involved, the method for evaluating vWF-cp activity had intra- and inter-batch coefficient of variation(CV) of 4.81% (n=8) and 8.63% (n=6), respectively. According to this method, the plasma vWF-cp activity in the 60 healthy adults was significantly higher than that in the CI patients [(86.53-/+17.49)% vs (77.15-/+16.72)%, P<0.05]. In TTP patients before plasma replacement, the vWF-cp activity was (9.06-/+7.17)% and increased significantly to (47.00-/+6.27)% 24 h after plasma replacement, respectively, but still significantly lower than that of healthy adults (P<0.01), whereas in the convalescent stage, the activity approached the normal level [(83.18-/+8.83)%, P>0.05].</p><p><b>CONCLUSIONS</b>According to the described method, which allows accurate vWF-cp activity measurement with good sensitivity, specificity and reproducibility, vWF-cp activity is lower in CI patients and even more so in TTP patients than that of healthy adults. Plasma replacement can effectively increase the vWF-cp activity in TTP patients.</p>
Полный текст:
1
База данных:
WPRIM
Основная тема:
Purpura, Thrombotic Thrombocytopenic
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Substrate Specificity
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Blood
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Cerebral Infarction
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Reproducibility of Results
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ADAM Proteins
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Enzyme Assays
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ADAMTS13 Protein
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Metabolism
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Methods
Пределы темы:
Animals
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Female
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Humans
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Male
Язык:
Zh
Журнал:
Journal of Southern Medical University
Год:
2007
Тип:
Article