Evaluation of Quality of Washed Platelets Stored in Platelet Additive Solutions / 대한수혈학회지
Korean Journal of Blood Transfusion
; : 67-76, 2017.
Article
в Ko
| WPRIM
| ID: wpr-34206
Ответственная библиотека:
WPRO
ABSTRACT
BACKGROUND: Because of a lack of substances for platelet (PLT) metabolism and preservation, normal saline (NS) washed PLTs can only be stored for short lengths of time. However, the use of platelet additive solutions (PAS) could help solve this problem. In this study, the in vitro quality of NS washed platelets (wPLTs) stored in two types of PAS were compared with those of wPLTs stored in NS. METHODS: Five units of NS washed apheresis platelets were pooled aseptically and separated into five aliquots for storage in NS only as well as T-PAS+ (Terumo BCT, Lakewood, CO, USA) and CompoSol PS (Fenwal, Lake Zurich, IL, USA) with or without 15 mM glucose. The parameters of wPLTs quality were assessed up to 48 hrs after washing and the whole experiment was repeated 10 times independently. RESULTS: wPLTs in two kinds of PAS had better quality than wPLTs in NS, and wPLTs in T-PAS+ showed better quality than those in CompoSol PS. PAS-stored wPLTs with added glucose maintained stable CD62P and Annexin V expression during storage, but exhibited increased lactate accumulation. Evaluation of in vitro quality revealed that all wPLTs had a rating of 4 immediately after washing. However, only T-PAS+-stored wPLTs with glucose maintained a rating of 4 up to 48 hrs of post-washing. CONCLUSION: Using PAS storage for wPLTs may be beneficial compared to NS. The results presented herein suggest that T-PAS+ containing glucose has the potential to extend storage time by up to 48-hours.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Blood Component Removal
/
Blood Platelets
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Blood Preservation
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In Vitro Techniques
/
Lakes
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Annexin A5
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Lactic Acid
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Glucose
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Metabolism
Язык:
Ko
Журнал:
Korean Journal of Blood Transfusion
Год:
2017
Тип:
Article