Alternatively spliced variants of constitutive androstane receptor in liver of mouse / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the existence of alternatively spliced variants of constitutive androstane receptor (CAR) in liver of mouse.</p><p><b>METHODS</b>The nucleotide from liver of mouse was purified and the CAR cDNA was amplified by PCR. The fragments of CAR cDNA were cloned to T vector and sequence analysis was performed.</p><p><b>RESULT</b>Various spliced variants of CAR in liver mouse were confirmed by DNA sequencing.</p><p><b>CONCLUSION</b>There are alternatively spliced variants in CAR, which are located in the ligand binding sequence of CAR.</p>