Study on rapid screening and identifying hepatotoxic compounds in toosendan fructus / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1820-1822, 2013.
Article
в Zh
| WPRIM
| ID: wpr-346492
Ответственная библиотека:
WPRO
ABSTRACT
Fluorescein diacetate-labeled HepG2 cells model and flouresence automatic microscopy screening assay were used for fast screening 23 components from Toosendan Fructus, in which 5 components showed significant toxicity on HepG2 cells. The 10 compounds in the 2 components were tentatively identified with LC-MS(n), and 3 of them (meliasenin B, trichilinin D and 1-O-tigloy-1-O-debenzoylohchinal) were prepared and identified. Further experiments showed that the 3 compounds displayed dose-dependent toxicity on HepG2 cells, suggesting that these compounds in Toosendan Fructus may cause hepatotoxicity.
Полный текст:
1
База данных:
WPRIM
Основная тема:
Drugs, Chinese Herbal
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Melia
/
Drug-Related Side Effects and Adverse Reactions
/
Toxicity
/
Hep G2 Cells
/
Fruit
/
Liver
Тип исследования:
Diagnostic_studies
/
Screening_studies
Пределы темы:
Humans
Язык:
Zh
Журнал:
China Journal of Chinese Materia Medica
Год:
2013
Тип:
Article