DDP-sensitivity-related genes in 10 lung cancer cell lines / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 418-421, 2008.
Article
в Zh
| WPRIM
| ID: wpr-357409
Ответственная библиотека:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To search for genes related to cisplatin (DDP) sensitivity in small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC) cell lines.</p><p><b>METHODS</b>The sensitivity of 4 SCLC lines and 6 NSCLC lines to DDP was evaluated by MTT assay. The expression of 1291 genes related to DDP-sensitivity in the 10 cell lines was measured by cDNA macroarray and the relationship between genes and DDP-sensitivity was analyzed.</p><p><b>RESULTS</b>20 genes were negatively related to DDP-sensitivity in the SCLC and NSCLC cell lines, including Metallothionein, Cathepsin B, TIMP1, TNF-R1, TGF beta-induced 68 000, Cathepsin L, Galectin-1, Annexin 11, PAI-1, IGFBP4, UPAR, Jagged, CD13, alpha 1 A-AR, EphA2 (Eck), APC, RhoC, Fibromodulin, GATA-6 and HSC 70, while only procoagula and MDM2 were positively related to DDP-sensitivity in the SCLC and NSCLC cell lines. 10 genes were negatively related to DDP-sensitivity in the SCLC cell lines, including VHL, MMP-7, Elongin A, GSK-3 beta, SLC, Galectin-3, integrin beta 5, moesin, IKK beta, and ETV 1, while only AT2 was positively related to DDP-sensitivity in the SCLC cell lines. 10 genes were negatively related to DDP-sensitivity in the NSCLC cell lines, including Clusterin, FG FR-2, Thrombospondin 1, HSP 32, Lactate dehydrogenase A, P300, Thymosin beta l0, CD81, C/EBP gamma, Rak, while only CaMKK and TPA were positively related to DDP-sensitivity in the NSCLC cell lines.</p><p><b>CONCLUSION</b>There were 45 genes related to DDP-sensitivity in 10 lung cancer cell lines. There were 22 co-expressed genes in both SCLC and NSCLC cell lines, and only 11 and 12 genes expressed in the SCLC and NSCLC cell lines, respectively.</p>
Полный текст:
1
База данных:
WPRIM
Основная тема:
Pathology
/
Pharmacology
/
Gene Expression Regulation, Neoplastic
/
Cisplatin
/
Carcinoma, Non-Small-Cell Lung
/
Drug Resistance, Neoplasm
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Cell Line, Tumor
/
Small Cell Lung Carcinoma
Тип исследования:
Diagnostic_studies
Пределы темы:
Humans
Язык:
Zh
Журнал:
Chinese Journal of Oncology
Год:
2008
Тип:
Article