Collapsin response mediator protein 5 accelerates neurite outgrowth in hippocampal neurons / 解剖学报
Acta Anatomica Sinica
; (6): 297-303, 2014.
Article
в Zh
| WPRIM
| ID: wpr-452002
Ответственная библиотека:
WPRO
ABSTRACT
Objective To investigate function of collapsin response mediator protein 5 ( CRMP5 ) on neurite outgrowth.Methods The CRMP5 eukaryotic expression vector was constructed and transfected into hippocampal neurons . The gene transfection, Real-time quantitative PCR and Western blotting were used to detect expression of CRMP 5 protein. The lapse-time imaging and neurite extraction were utilized to show neurite outgrowth and differentiation and 3 double-pored were performed, compared with the vector without CRMP5 gene.Results It was successful to construct the CRMP5 eukaryotic expression vector with an EGFP tag .The lipofectamine effectually transfected CRMP 5 into cultured neurons , and the CRMP5 protein was expressed successfully more than the control cells .CRMP5 protein was abundant in cell body , initiation and end of neurites .Overexpression of CRMP5 in neuronal cells significantly promoted outgrowth neurites , and led to the formation of longer neurites with more branches .Accompanying rapid outgrowth of neurites , branches from original neurites were contributed to form a network .The results of neurite length and extraction showed that neurons overexpressing CRMP5 were possessed more and longer neurites (P<0.01), compared with control cells .Conclusion The results suggest that CRMP5 accelerates not only axonal growth but also branching .
Полный текст:
1
База данных:
WPRIM
Язык:
Zh
Журнал:
Acta Anatomica Sinica
Год:
2014
Тип:
Article