Use ITS gene sequence analysis to identify dematiaceous fungi / 实用医学杂志
The Journal of Practical Medicine
; (24): 2493-2496, 2014.
Article
в Zh
| WPRIM
| ID: wpr-455192
Ответственная библиотека:
WPRO
ABSTRACT
Objectives Use ITS gene sequence analysis to identify 15 strains of dematiaceous fungi , to learn the types of pathogenic strains and clinical treatment. Methods By observing the colony morphology and microscope morphological of the dematiaceous fungi isolated from superficial mycoses , and identified by ITS gene sequence analysis. Results 15 strains were identified by morphological observation as dematiaceous fungi.The amplified bands were identified by Tanon-3500 gel imaging system between 500 ~ 700 bp. Blast sequencing results show that 2 strains Alternaria alternate , 2 strains Cladosporium sphaerospermum. 2 strains Exophiala dermatitis, 1 strains Cladosporium cladosporioides, Curvularia lunata, Talaromyces rugulosus, Phaeobotryon cupressi, Cladosporium tenuissimum, Fonseceea pedrosoi, Exophiala werneckii, Exophiala oligosperma and Fonsecaea monophora. Conclusion ITS gene sequence analysis can identify dematiaceous fungi effectively , avoided undetected and misdiagnose cause by the lack of clinical experience.
Полный текст:
1
База данных:
WPRIM
Язык:
Zh
Журнал:
The Journal of Practical Medicine
Год:
2014
Тип:
Article