Evaluation and improvement of anti-interference performance of D-3-hydroxybutyrate detection reagent / 国际检验医学杂志
International Journal of Laboratory Medicine
; (12): 1991-1992, 2014.
Article
в Zh
| WPRIM
| ID: wpr-455230
Ответственная библиотека:
WPRO
ABSTRACT
Objective To evaluate the anti-interference performance of D-3-hydroxybutyrate (D3H ) reagent kit by using the NCCLS document EP7-A2 and to improve its anti-interference performance .Methods The anti-interference evaluation test of D3H kit was performed according to NCCLS document EP7-A2 and its anti-interference performance was improved by adding vitamin C oxidase .Results 342 mol/L free bilirubin ,342 mol/L conjugated bilirubin ,1 450 FTU chyle and 5 g/L hematoglobin had no inter-ference on the high concentration D3H detection ,0 .03 g/L vitamin C interfered with the low and high concentration D3H detection . The dosage-effect experiment results showed that vitamin C produced the linear positive interference effect on D 3H detection . Adding 2 500 U/L of vitamin C oxidase could antagonize the interference of vitamin C on detection .Conclusion Vitamin C can in-terfere with the D3H detection and adding vitamin C oxidase in reagent can antagonize its interference .
Полный текст:
1
База данных:
WPRIM
Тип исследования:
Diagnostic_studies
Язык:
Zh
Журнал:
International Journal of Laboratory Medicine
Год:
2014
Тип:
Article