Study on proliferation in vitro and mineral composition of Phyllathus amarus by combined activated neutron analysis

ABSTRACT

The conservation of precious source of medicinal herb from traditional medicine phyllathus amarus (= P. niruri L.) was effectively conducted by in vitro propagation. The process of in vitro propagation of this culture consists of main following stages: (1) Start budding: suitable materials are meristems, apical buds and auxiliary buds; Rapid propagation: The medium for highest propagation coefficient is MS; creating complete plantlets: rapid root differentiation with good quality roots is observed in the medium MS+ NAA; in nurseries: the suitable substrate for ex-vitro stage is black sandy soils; (2) The medium able to form potassium at highest rate is MS + 2.4D; (3) The analysis of mineral compositions shows low contents of heavy metals and rare earth elements (REE), but considerably high in potassium, that may be due to diuretic activity of P. amarus.