Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection
The Korean Journal of Parasitology
; : 375-380, 2016.
Article
в En
| WPRIM
| ID: wpr-50648
Ответственная библиотека:
WPRO
ABSTRACT
Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Enzyme-Linked Immunosorbent Assay
/
Immunoassay
/
Serologic Tests
/
Sensitivity and Specificity
/
Angiostrongylus cantonensis
/
Diagnosis
/
Limit of Detection
/
Angiostrongylus
/
Methods
/
Antibodies, Monoclonal
Тип исследования:
Diagnostic_studies
Пределы темы:
Adult
/
Humans
Язык:
En
Журнал:
The Korean Journal of Parasitology
Год:
2016
Тип:
Article