Association and function study of tumor necrosis factor receptorⅡ position 196 polymorphism with systemic lupus erythematosus / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1999.
Article
в Zh
| WPRIM
| ID: wpr-525794
Ответственная библиотека:
WPRO
ABSTRACT
AIM:
To investigate the association of gene polymorphism at position 196 of tumor necrosis factor receptor Ⅱ (TNFRⅡ) with systemic lupus erythematosus (SLE) in Chinese, and establish recombinant retroviral vector to analyze the function of the TNFRⅡ 196M/R.METHODS:
The genotype at position 196 of TNFRⅡ was determined by PCR-RFLP in 106 SLE patients and 119 healthy controls in china. Human TNFRⅡ196M cDNA were amplified by PCR and cloned into PMD18-T vector. Then, PMD18-TNFRⅡ196R was induced by site-directed mutagenesis. The recombinant T vector, PMD18-TNFRⅡ196M and PMD18-TNFRⅡ196R, were subcloned into retroviral vector PLXSN. Both normal and variant were transfected into rat mesangial cell. The effects of TNF? on production of sTNFRⅡ and IL-6 were study by ELISA.RESULTS:
(1) The frequency of TNFRⅡ196R allele was significantly higher than those in controls (35.2% vs 14.3%, P
Полный текст:
1
База данных:
WPRIM
Язык:
Zh
Журнал:
Chinese Journal of Pathophysiology
Год:
1999
Тип:
Article