Inhibitory effect of Yichuanping capsule (易喘平胶囊) on the airway mucus overproduction in asthmatic mice / 中国中西医结合急救杂志

ABSTRACT

Objective: To evaluate the inhibitory effect of Yichuanping capsule (易喘平胶囊) on the airway mucus overproduction in asthmatic mice and its mechanism. Methods: Thirty-two male BALB/c mice were randomly divided into normal control group, asthma group, dexamethasone group and Yichuanping capsule (YC) group (n=8 in each group). Asthmatic mouse model was established by intraperitoneal injection of ovalbumin. The number of goblet cells and secretion of mucus were detected with periodic acid-Schiff (PAS) staining, and mucus protein MUC5AC mRNA was detected with reverse transcription-polymerase chain reaction (RTCD*2PCR). Number of total cells and eosinophil in bronchoalveolar lavage fluid (BALF) were counted with special staining. The contents of interleukinCD*24 (ILCD*24) and ?CD*2interferon (IFNCD*2?) in lung tissue were assessed by enzyme-linked immunoadsorbent assay (ELISA). Results: Compared with asthma group, the number of goblet cells, MUC5AC mRNA levels and secretion of mucus in airway were reduced in dexamethasone group and YC group(all P