Effects of Wortmannin combined with mitomycin C on proliferation and apoptosis of HepG-2 cells / 第三军医大学学报
Journal of Third Military Medical University
; (24)2003.
Article
в Zh
| WPRIM
| ID: wpr-567989
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WPRO
ABSTRACT
Objective To investigate the effects of Wortmannin combined with mitomycin C (MMC) on the proliferation,apoptosis and expressions of related genes Cyclin D1 and Bad in liver cancer cell line HepG-2. Methods HepG-2 cells (104/ml) were treated with 0,25,50,100,200 or 400 nmol/L Wortmannin,0,1.75,3.75,7.5,15 or 30 nmol/L MMC,or combined concentrations (0,26.875,53.75,107.5,215 or 430 nmol/L) respectively. Cell suppression rate were detected by MTT assay. Nucleus apoptosis of Hep-G-2 cells were examined by Hochest 33258 fluorescent staining. Change of cell cycle and apoptosis in HepG-2 cells were detected by flow cytometry (FCM). Expressions of PI3K/Akt signaling transduction pathway related genes and proteins were tested by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. Results The inhibition ratio of cell proliferation was significant higher in combined groups than in either single groups (P
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1
База данных:
WPRIM
Язык:
Zh
Журнал:
Journal of Third Military Medical University
Год:
2003
Тип:
Article