Effects of hyperglycemia on proliferation,secretion function and expression of endoplasmic reticulum stress related molecules of pancreatic ? cell line / 上海第二医科大学学报
Article
в Zh
| WPRIM
| ID: wpr-640887
Ответственная библиотека:
WPRO
ABSTRACT
Objective To investigate the effects of chronic hyperglycemia on the proliferation,secretion function and expression of endoplasmic reticulum stress(ERS) related molecules in pancreatic ? cell line MIN6. Methods MIN6 cells were treated with different concentrations of glucose(5.6,25 and 33.3mmol/L) and were harvested at indicated time(24h and 96h) for examinations.Cell proliferation was tested using CCK-8 solution,insulin and proinsulin secretion function was determined by ELISA,and mRNA expression of ERS related molecules(Total XBP-1,Spliced XBP-1) and protein expression of phosphorylated IRE1? were detected by Real-time PCR and Western blotting,respectively. Results After treatment with hyperglycemia for 96h, cell proliferation was significantly lower than that treated for 24h(P
Полный текст:
1
База данных:
WPRIM
Язык:
Zh
Журнал:
Journal of Shanghai Jiaotong University(Medical Science)
Год:
2006
Тип:
Article