Protease-Activated Receptors 2-Antagonist Suppresses Asthma by Inhibiting Reactive Oxygen Species-Thymic Stromal Lymphopoietin Inflammation and Epithelial Tight Junction Degradation
Allergy, Asthma & Immunology Research
; : 560-571, 2019.
Article
в En
| WPRIM
| ID: wpr-762138
Ответственная библиотека:
WPRO
ABSTRACT
PURPOSE: Protease-activated receptor 2 (PAR2) reportedly triggers the immune response in allergic asthma. We aimed to investigate the mechanism on allergic inflammation mediated by PAR2. METHODS: Human lung epithelial cells (A549 cells) were used for in vitro, and the German cockroach extract (GCE)-induced mouse model was developed for in vivo studies. RESULTS: In A549 cells, the levels of reactive oxygen species (ROS) and thymic stromal lymphopoietin (TSLP) were significantly increased by GCE treatment, but were suppressed by PAR2-antagonist (PAR2-ant) or N-acetylcysteine (NAC) treatment. Claudin-1 was degraded by GCE, and was restored by PAR2-ant or NAC in the cells. In the mouse model, the clinical appearance including bronchial hyperresponsiveness, bronchoalveolar lavage fluid analysis and total immunoglobulin E were significantly suppressed by PAR2-ant or NAC. Moreover, TSLP levels in the lung were suppressed by the same treatments in the lung. Claudin-1 was also degraded by GCE, and was restored by PAR2-ant or NAC. CONCLUSIONS: ROS generation and epidermal tight junction degradation are triggered by protease, followed by the induction of TSLP in allergic asthma. Our findings could suggest that PAR2-ant or anti-oxidants could be considered for allergic diseases as preventive alternatives.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Oxygen
/
Acetylcysteine
/
Asthma
/
In Vitro Techniques
/
Immunoglobulin E
/
Immunoglobulins
/
Bronchoalveolar Lavage Fluid
/
Reactive Oxygen Species
/
Tight Junctions
/
Blattellidae
Тип исследования:
Prognostic_studies
Пределы темы:
Animals
/
Humans
Язык:
En
Журнал:
Allergy, Asthma & Immunology Research
Год:
2019
Тип:
Article