Relationship between expression level of sex-determining region Y box 9 and metastasis of oral squamous cell carcinoma / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 688-693, 2018.
Article
в Zh
| WPRIM
| ID: wpr-807460
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WPRO
ABSTRACT
Objective@#To investigate the effects of sex-detemining region Y box9 (SOX9) expression levels on the proliferation, migration and metastasis in oral squamous cell carcinoma (OSCC).@*Methods@#A total of 74 OSCC pathological specimens were collected from Shanghai OSCC Tissue and Biological Informations Bank, and clinicopathological information of these specimens were collected. Immunohistochemistry assay was used to examine the expression levels of SOX9 in OSCC and to analyze their relationship with clinicopathological features. Cell counting kit-8 assay and cloning formation was used to observe the relationship between the expression levels of SOX9 and the proliferation of OSCC. Transwell experiment and scratch test were used to detect the difference of the ability of OSCC in cell lines with different expression levels of SOX9.@*Results@#The risk of lymph node metastasis in patients with high expression of SOX9 was significantly increased (P=0.010). In the Transwell experiment, the number of HN6 cells (671.0±57.4, P=0.000) migrated to the lower chamber more than that of CAL27 cells (172.0±13.9). In the scratch experiment, HN6 cells [0 h (93.7±2.1) μm; 6 h (56.7±2.5) μm; 12 h (29.7±3.1) μm] migrated faster than CAL27 [0 h (93.7±1.5) μm; 6 h (78.0±2.0) μm; 12 h (42.0±3.0) μm](P<0.05). The migration ability of the cell line (HN6) with high-expression of SOX9 was significantly higher than that in cell line (CAL27) with low-expression SOX9 (P<0.05). The expression levels of SOX9 in OSCC were no significant on cell proliferation (P>0.05).@*Conclusions@#High expression of SOX9 can promote the migration and lymph node metastasis of OSCC. SOX9 is a candidate gene target for the diagnosis and intervention of lymph node metastasis in OSCC.
Полный текст:
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База данных:
WPRIM
Язык:
Zh
Журнал:
Chinese Journal of Stomatology
Год:
2018
Тип:
Article