DT-13, a saponin of dwarf lilyturf tuber, exhibits anti-cancer activity by down-regulating C-C chemokine receptor type 5 and vascular endothelial growth factor in MDA-MB-435 cells / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 24-29, 2014.
Article
в En
| WPRIM
| ID: wpr-812313
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WPRO
ABSTRACT
AIM@#To investigate the anticancer activity of DT-13 under normoxia and determine the underlying mechanisms of action.@*METHODS@#MDA-MB-435 cell proliferation, migration, and adhesion were performed to assess the anticancer activity of DT-13, a saponin from Ophiopogon japonicus, in vitro. In addition, the effects of DT-13 on tumor growth and metastasis in vivo were evaluated by orthotopic implantation of MDA-MB-435 cells into nude mice; mRNA levels of vascular endothelial growth factor (VEGF), C-C chemokine receptor type 5 (CCR5) and hypoxia-inducible factor 1α (HIF-1α) were evaluated by real-time quantitative PCR; and CCR5 protein levels were detected by Western blot assay.@*RESULTS@#At 0.01 to 1 μmol·L(-1), DT-13 inhibited MDA-MB-435 cell proliferation, migration, and adhesion significantly in vitro. DT-13 reduced VEGF and CCR5 mRNAs, and decreased CCR5 protein expression by down-regulating HIF-1α. In addition, DT-13 inhibited MDA-MB-435 cell lung metastasis, and restricted tumor growth slightly in vivo.@*CONCLUSION@#DT-13 inhibited MDA-MB-435 cell proliferation, adhesion, and migration in vitro, and lung metastasis in vivo by reducing VEGF, CCR5, and HIF-1α expression.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Saponins
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Breast Neoplasms
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Drugs, Chinese Herbal
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Cell Adhesion
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Cell Movement
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Chemistry
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Receptors, CCR5
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Liriope Plant
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Plant Tubers
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Cell Line, Tumor
Пределы темы:
Animals
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Female
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Humans
Язык:
En
Журнал:
Chinese Journal of Natural Medicines (English Ed.)
Год:
2014
Тип:
Article