Simultaneous determination of four components of Shuganning Injection in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2626-2633, 2020.
Article
в Zh
| WPRIM
| ID: wpr-828036
Ответственная библиотека:
WPRO
ABSTRACT
This study aims to reveal the pharmacokinetics of Shuganning Injection in normal rats. In this experiment,ultra-high performance liquid chromatography-electrospray-tandem mass spectrometry( UPLC-ESI-MS/MS) was used to establish an analytical method for simultaneous determination of chlorogenic acid,gardenioside,oroxylin A and baicalin in rat plasma. Then,the non-compartmental model( NCA) in Phoenix WinN onL in 6. 4 software was used to fit pharmacokinetic parameters. The methodological validation showed that the linear relationship of the components in rat plasma samples were good( r>0. 995). The recovery rate and matrix effect of plasma samples with low,middle and high concentration were 79. 14%-101. 4%. The intra-day and inter-day precision,accuracy and stability meet the requirements of biological sample analysis. The half-life( t1/2) of chlorogenic acid,gardenioside,oroxylin A did not change significantly and the area under blood concentration-time curve( AUC0-t) is proportional to the dose,which suggested that three components showed a linear kinetic characteristics,but baicalin showed nonlinear kinetic characteristics. Moreover,the retention time of each component in rats was short. The established UPLC-MS/MS quantitative analysis method is rapid,sensitive and accurate,which can be used for the determination of chlorogenic acid,gardenioside,oroxylin A and baicalin in rat plasma and pharmacokinetic study of Shuganning Injection.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Plasma
/
Chlorogenic Acid
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Chromatography, Liquid
/
Rats, Sprague-Dawley
/
Tandem Mass Spectrometry
Тип исследования:
Prognostic_studies
Пределы темы:
Animals
Язык:
Zh
Журнал:
China Journal of Chinese Materia Medica
Год:
2020
Тип:
Article