Assessment of the Sensitivity and Specificity of the Established Real-time Quaking-induced Conversion (RT-QuIC) Technique in Chinese CJD Surveillance / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 620-622, 2020.
Article
в En
| WPRIM
| ID: wpr-828972
Ответственная библиотека:
WPRO
ABSTRACT
Real-time quaking-induced conversion (RT-QuIC) assay is a newly established PrP -detecting method. The development of RT-QuIC improves the diagnosis of sporadic Creutzfeldt-Jakob disease (sCJD), showing good sensitivity and specificity in many countries when the method was used in cerebrospinal fluid (CSF) samples. However, in China, the sensitivity and specificity of RT-QuIC has yet to be determined due to the lack of definitive diagnosis samples. Recently, 30 definitive sCJD and 30 non-CJD diagnoses were evaluated by RT-QuIC assay. In the 30 sCJD CSF samples, 29 showed positive results. By contrast, all the non-CJD samples were negative. The sensitivity and specificity of our RT-QuIC assay were 96.67% and 100%, respectively, and are comparable to other published data. Results can provide a fundamental basis for the usage of RT-QuIC assay in CJD surveillance in China.
Полный текст:
1
База данных:
WPRIM
Основная тема:
Cerebrospinal Fluid
/
China
/
Creutzfeldt-Jakob Syndrome
/
Sensitivity and Specificity
/
PrPSc Proteins
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Diagnosis
/
Diagnostic Tests, Routine
/
Methods
Тип исследования:
Diagnostic_studies
/
Screening_studies
Пределы темы:
Humans
Страна как тема:
Asia
Язык:
En
Журнал:
Biomedical and Environmental Sciences
Год:
2020
Тип:
Article