Study on the Extraction Technology of Fructus Gleditisae Abnormalis and GC-MS Analysis of Extracted Com- ponents / 中国药房
China Pharmacy
; (12): 2469-2472, 2020.
Article
в Zh
| WPRIM
| ID: wpr-829352
Ответственная библиотека:
WPRO
ABSTRACT
OBJECTIVE:To establish and optimize a extraction method of Fructus Gleditsiae Abnormallis ,and to analyze and identify chemical components of the extract simultaneously. METHODS :Fructus Gleditsiae Abnormallis was extracted with CO 2 supercritical fluid extraction (SFE)method. Based on single factor tests ,using extraction yield as index ,extraction temperature , extraction pressure and extraction time as investigation factors ,SFE technology was optimized with orthogonal test ,and validation test was performed. Chemical components in the extract were identified by GC-MS. Relative percentage of each component was calculated with area normalization method. RESULTS :The optimal SFE extraction technology of Fructus Gleditsiae Abnormallis was extraction temperature of 60 ℃,extraction pressure of 300 MPa and pression time of 15 min. Average extraction of 3 times of validation tests was 1.73%(RSD=1.78%,n=3). The 48 components in the extracts of Fructus Gleditsiae Abnormallis were identified,which accounted for 98.31% of the total amount of the extracts. The extracts of Fructus Gleditisae Abnormalis mainly included organic acids ,accounting for 36.99%,followed by alkaloids ,accounting for 12.59% in total. Main components were palmitic acid (16.62%),oleic acid (14.12%),N-aminotetrahydropyrrole(9.79%),2,6-dimethyloctane-1,7-dien-3-ol(5.95%), tetrahydropyran(3.83%),vanillin(3.39%),etc. CONCLUSIONS :SFE method of Fructus Gleditisae Abnormalis is established successfully,and the extract is mainly organic acids.
Полный текст:
1
База данных:
WPRIM
Тип исследования:
Prognostic_studies
Язык:
Zh
Журнал:
China Pharmacy
Год:
2020
Тип:
Article