HPLC fingerprint of Moutan Cortex / 中草药
Chinese Traditional and Herbal Drugs
; (24): 900-904, 2013.
Article
в Zh
| WPRIM
| ID: wpr-855421
Ответственная библиотека:
WPRO
ABSTRACT
Objective:
To establish the fingerprint of Moutan Cortex by HPLC.Methods:
The chromatographic fingerprint was obtained with Dikma Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) and gradient eluted with acetonitrile and water (with 0.085% phosphoric acid). The column temperature was maintained at 35 °C, and the flow rate was 1 mL/min. The detection wavelength was 254 (0-21.5 min), 230 (21.5-45 min), and 254 nm (45-60 min). The injection volume was 20 μL. The peaks were identified by LC-MS/MS.Results:
The method had good precision, stability, and repeatability. Eighteen chromatographic peaks among 20 common peaks were identified by LC-MS/MS. Ten batches of Moutan Cortex were determined, and the similarities were above 0.96.Conclusion:
The study lays the foundation for the full quality evaluation of Moutan Cortex.
Полный текст:
1
База данных:
WPRIM
Язык:
Zh
Журнал:
Chinese Traditional and Herbal Drugs
Год:
2013
Тип:
Article