HPLC fingerprint of Moutan Cortex / 中草药

ABSTRACT

Objective: To establish the fingerprint of Moutan Cortex by HPLC. Methods: The chromatographic fingerprint was obtained with Dikma Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) and gradient eluted with acetonitrile and water (with 0.085% phosphoric acid). The column temperature was maintained at 35 °C, and the flow rate was 1 mL/min. The detection wavelength was 254 (0-21.5 min), 230 (21.5-45 min), and 254 nm (45-60 min). The injection volume was 20 μL. The peaks were identified by LC-MS/MS. Results: The method had good precision, stability, and repeatability. Eighteen chromatographic peaks among 20 common peaks were identified by LC-MS/MS. Ten batches of Moutan Cortex were determined, and the similarities were above 0.96.Conclusion: The study lays the foundation for the full quality evaluation of Moutan Cortex.