Inhibitory mechanism of oridonin on G2/M phase arrest in SGC-7901 cell growth / 中草药
Chinese Traditional and Herbal Drugs
; (24): 2024-2026, 2010.
Article
в Zh
| WPRIM
| ID: wpr-855731
Ответственная библиотека:
WPRO
ABSTRACT
Objective: To investigate the effect of oridonin on G2/M phase arrest of human stomach cancer SGC-7901 cell growth in vitro and its molecular mechanism. Methods: The inhibition of oridonin on SGC-7901 cells was detected by MTT assay. Effect of oridonin on the phase distribution of SGC-7901 cell cycle was observed by flow cytometry (FCM). Western blotting was used to analyze the expression of Cdk1 and CyclinB1. Results: IC50 of oridonin on SGC-7901 cells was 15. 64 μmol/L. The effect of oridonin on SGC-7901 cells presented the increasing percentages of cells in G2/M phase as the concentration of oridonin increased. With the increase of oridonin dose, the expression of Cdk1 and CyclinB1 proteins was remarkably decreased. Conclusion: Oridonin can down-regulate the expression of Cdk1 and CyclinB1, which may be the mechanism of arresting SGC-7901 cells in G2/M phase.
Полный текст:
1
База данных:
WPRIM
Язык:
Zh
Журнал:
Chinese Traditional and Herbal Drugs
Год:
2010
Тип:
Article