Progress in the construction and screening of random mutation library / 生物工程学报
Chinese Journal of Biotechnology
; (12): 163-177, 2021.
Article
в Zh
| WPRIM
| ID: wpr-878551
Ответственная библиотека:
WPRO
ABSTRACT
Directed evolution is a cyclic process that alternates between constructing different genes and screening functional gene variants. It has been widely used in optimization and analysis of DNA sequence, gene function and protein structure. It includes random gene libraries construction, gene expression in suitable hosts and mutant libraries screening. The key to construct gene library is the storage capacity and mutation diversity, to screen is high sensitivity and high throughput. This review discusses the latest advances in directed evolution. These new technologies greatly accelerate and simplify the traditional directional evolution process and promote the development of directed evolution.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Base Sequence
/
Proteins
/
Gene Library
/
Directed Molecular Evolution
/
Mutation
Тип исследования:
Clinical_trials
/
Diagnostic_studies
/
Screening_studies
Язык:
Zh
Журнал:
Chinese Journal of Biotechnology
Год:
2021
Тип:
Article