Optimization of expression conditions and determination the proteolytic activity of codon-optimized SARS-CoV-2 main protease in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1334-1345, 2021.
Article
в Zh
| WPRIM
| ID: wpr-878635
Ответственная библиотека:
WPRO
ABSTRACT
The main protease (Mpro) of SARS-CoV-2 is a highly conserved and mutation-resistant coronaviral enzyme, which plays a pivotal role in viral replication, making it an ideal target for the development of novel broad-spectrum anti-coronaviral drugs. In this study, a codon-optimized Mpro gene was cloned into pET-21a and pET-28a expression vectors. The recombinant plasmids were transformed into E. coli Rosetta(DE3) competent cells and the expression conditions were optimized. The highly expressed recombinant proteins, Mpro and Mpro-28, were purified by HisTrapTM chelating column and its proteolytic activity was determined by a fluorescence resonance energy transfer (FRET) assay. The FRET assay showed that Mpro exhibits a desirable proteolytic activity (25 000 U/mg), with Km and kcat values of 11.68 μmol/L and 0.037/s, respectively. The specific activity of Mpro is 25 times that of Mpro-28, a fusion protein carrying a polyhistidine tag at the N and C termini, indicating additional residues at the N terminus of Mpro, but not at the C terminus, are detrimental to its proteolytic activity. The preparation of active SARS-CoV-2 Mpro through codon-optimization strategy might facilitate the development of the rapid screening assays for the discovery of broad-spectrum anti-coronaviral drugs targeting Mpro.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Peptide Hydrolases
/
Codon
/
Cysteine Endopeptidases
/
Viral Nonstructural Proteins
/
Escherichia coli
/
SARS-CoV-2
/
COVID-19
Пределы темы:
Humans
Язык:
Zh
Журнал:
Chinese Journal of Biotechnology
Год:
2021
Тип:
Article