Study on the stability of SARS-CoV-2 at different temperatures / 上海预防医学
Shanghai Journal of Preventive Medicine
; (12): 818-823, 2021.
Article
в Zh
| WPRIM
| ID: wpr-887144
Ответственная библиотека:
WPRO
ABSTRACT
Objective:To observe the stability of severe acute respiratory syrdrome coronavirus (SARS-CoV-2) in cell cultures at different temperatures so as to provide basic data and scientific basis for the research and control of COVID-19 epidemic. Methods:The Vero E6 cells inoculated with SARS-CoV-2. According to TCID50, SARS-CoV-2 with different dilution (10-1, 10-3, 10-5, 10-6)were stored at 37 °C, 22.5 °C, and 4 °C for one to seven days, and then infectious titer was determined by micro cytopathogenic effect assay, observing cytopathic effect (CPE), and real-time fluorescence quantitative testing. Results:SARS-CoV-2 was stable under 4 °C. The infectivity of high concentration (10-1 dilution) under 22.5 °C for seven days gradually decreased, while lower concentration completely lost infectivity after one day. The virus lost infectivity when stored at 37 °C for more than one day. Conclusion:SARS-CoV-2 is highly stable at 4 °C, sensitive to heat, and related to virus concentration.
Полный текст:
1
База данных:
WPRIM
Язык:
Zh
Журнал:
Shanghai Journal of Preventive Medicine
Год:
2021
Тип:
Article