Research progress in the rapid preparation of monoclonal antibodies of mouse hybridoma / 生物工程学报
Chinese Journal of Biotechnology
; (12): 2293-2306, 2021.
Article
в Zh
| WPRIM
| ID: wpr-887797
Ответственная библиотека:
WPRO
ABSTRACT
Mouse hybridoma monoclonal antibody is the most commonly used antibody in immunology because of its stable source, easy preparation in later stage and high yield. The traditional time-consuming and laborious hybridoma preparation technology could not meet the growing market demand. In this paper, we describe the rapid preparation techniques involved in antigen design and screening, B cell enrichment and screening, transgenic myeloma cells, fusion technology improvement, positive hybridoma cell screening and rapid detection of monoclonal antibody performance, to provide a reference for rapid preparation of mouse hybridoma monoclonal antibody.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
B-Lymphocytes
/
Hybridomas
/
Antibodies, Monoclonal
/
Antigens
Пределы темы:
Animals
Язык:
Zh
Журнал:
Chinese Journal of Biotechnology
Год:
2021
Тип:
Article