Effect of 17β-estradiol on the proliferation of condylar chondrocytes / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 651-657, 2021.
Article
в En
| WPRIM
| ID: wpr-921387
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ABSTRACT
OBJECTIVES@#To study the effects of 17β-estradiol (E2) on the regulation of the proliferation of condylar chondrocytes and provide a preliminary discussion on the role of phosphorylate-mammalian target of rapamycin (p-mTOR) in this regulatory process.@*METHODS@#Condylar chondrocytes were isolated from 6-week-old female rats for primary culture. Drug treatment with different concentrations of E2 and/or rapamycin (RAPA) was carried out on second-generation cells. Cell Counting Kit 8 was used to measure the cell viability of condylar chondrocytes after culture for 24, 48, or 72 h, and reverse transcription-polymerase chain reaction (RT-PCR) was applied to detect the relative gene expression of estrogen receptor alpha (ERα), estrogen receptor beta (ERβ), collagen type Ⅱ (COLⅡ), autophagy-related gene 6 (Beclin-1), and autophagy-related gene 5 (ATG-5). Western blot was employed to determine the relative protein expression of ERα, ERβ, Beclin-1, lipid-modified light chain 3B (LC3-Ⅱ), and p-mTOR.@*RESULTS@#E2 could significantly promote the proliferation of chondrocytes cultured @*CONCLUSIONS@#At a concentration of 10
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Phosphorylation
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Autophagy
/
Chondrocytes
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Estrogen Receptor alpha
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Estrogen Receptor beta
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Cell Proliferation
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Estradiol
Пределы темы:
Animals
Язык:
En
Журнал:
West China Journal of Stomatology
Год:
2021
Тип:
Article