Development of a motor neurons-specific fluorescence reporter system / 生物工程学报
Chinese Journal of Biotechnology
; (12): 4095-4101, 2021.
Article
в Zh
| WPRIM
| ID: wpr-921490
Ответственная библиотека:
WPRO
ABSTRACT
Human induced pluripotent stem cells (hiPSCs) have the potential to differentiate into multiple cell types. Motor neurons (MNs) differentiated from hiPSCs are important models of many motor neuron diseases. To simplify the identification of MNs, lentivirus vectors were used to transfer MNs-specific promoter HB9 and red fluorescent protein (RFP) gene into hiPSCs-derived human neural stem cells (hNSCs). Stable positive cells hNSCs-HB9-RFP-Puro were obtained after antibiotic selection. Subsequently, the positive cell line was infected with lentiviruses LV-Ngn2-Sox11-GFP and LV-Isl1-Lhx3-Hygro, which overexpressed the MNs differentiation transcription factor, and differentiated to MNs directly. Differentiated mature MNs showed neuron-like structure, expressed RFP and neuron-related markers β-tubulin and choline acetyltransferase (ChAT) under the control of the MNs-specific promoter HB9. The fluorescence reporter system provides a visual method for directed differentiation and identification of MNs, and may promote the applications of MNs in disease models and drug screening.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Transcription Factors
/
Cell Differentiation
/
Induced Pluripotent Stem Cells
/
Fluorescence
/
Motor Neurons
Тип исследования:
Prognostic_studies
Пределы темы:
Humans
Язык:
Zh
Журнал:
Chinese Journal of Biotechnology
Год:
2021
Тип:
Article