Ferulic acid enhances insulin secretion by potentiating L-type Ca2+ channel activation / 中西医结合学报
Journal of Integrative Medicine
; (12): 99-105, 2023.
Article
в En
| WPRIM
| ID: wpr-971647
Ответственная библиотека:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of ferulic acid, a natural compound, on pancreatic beta cell viability, Ca2+ channels, and insulin secretion.@*METHODS@#We studied the effects of ferulic acid on rat insulinoma cell line viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide viability assay. The whole-cell patch-clamp technique and enzyme-linked immunosorbent assay were also used to examine the action of ferulic acid on Ca2+ channels and insulin secretion, respectively.@*RESULTS@#Ferulic acid did not affect cell viability during exposures up to 72 h. The electrophysiological study demonstrated that ferulic acid rapidly and concentration-dependently increased L-type Ca2+ channel current, shifting its activation curve in the hyperpolarizing direction with a decreased slope factor, while the voltage dependence of inactivation was not affected. On the other hand, ferulic acid have no effect on T-type Ca2+ channels. Furthermore, ferulic acid significantly increased insulin secretion, an effect inhibited by nifedipine and Ca2+-free extracellular fluid, confirming that ferulic acid-induced insulin secretion in these cells was mediated by augmenting Ca2+ influx through L-type Ca2+ channel. Our data also suggest that this may be a direct, nongenomic action.@*CONCLUSION@#This is the first electrophysiological demonstration that acute ferulic acid treatment could increase L-type Ca2+ channel current in pancreatic β cells by enhancing its voltage dependence of activation, leading to insulin secretion.
Key words
Полный текст:
1
База данных:
WPRIM
Основная тема:
Calcium
/
Coumaric Acids
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Insulin-Secreting Cells
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Insulin Secretion
/
Insulin
Пределы темы:
Animals
Язык:
En
Журнал:
Journal of Integrative Medicine
Год:
2023
Тип:
Article